Other

Part:BBa_K5452106:Design

Designed by: Bianca Henriques de Oliveira   Group: iGEM24_USP-EEL-Brazil   (2024-09-27)


Untranslated regions (3’ UTR) located in the pep4 protease locus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 780
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 334


Design Notes

We chose the pep4 protease gene because its knockout does not interfere with yeast growth (Schoborg, Clark, Choudhury, C. Eric Hodgman, & Jewett, 2016).



Source

This part can be found in the genome of the Saccharomyces cerevisiae

References